Figure 5.

Barley cer Mutants Have Mutations in β-Diketone Biosynthesis Genes DMP, DMH, and DMC.

(A) A screen of barley cer mutants revealed eight mutants with either PTC or deletions in the β-diketone genes DMP, DMH, and DMC. ^acer-q.56 and cer-q.82 have the same mutation in the DMH gene; hence, it cannot be determined if these are two different mutants.

(B) to (D) RT-qPCR analysis of the gene cluster in wild-type bonus and cer-cqu.724 triple mutant supports a deletion in the genetic region comprising DMP, DMH, and DMC. Relative expression is shown for DMP (B), DMH (C), and DMC (D). Values indicate the mean ± sd of n ≥ 3 biological replicates. **P < 0.005 and ***P < 0.001