Fig. 6.

Effect of ErbB2 inhibitor on alcohol-induced activation of ErbB2, p38γ MAPK and SAP97. a MCF7-ErbB2 cells were pretreated with DMSO or AG825 (10 μM) for 2 h, followed by alcohol exposure (0 or 200 mg/dl) for indicated times. The phosphorylation of ErbB2 or p38γ was determined by immunoblotting. b The effect of alcohol on the phosphorylation of SAP97 at serine/threonine sites was determined by immunoprecipitation. Cell lysates were IP with an anti-SAP97 antibody and then IB with an antibody directed against phosphorylated serine/threonine. c MCF7-ErbB2 cells were pretreated with DMSO or AG825 (10 μM) for 2 h, followed by alcohol exposure (0 or 200 mg/dl) for indicated times. Equal amount of protein were IP with an anti-p38α/β antibody, then IB with an anti-pan-phosphorylated p38 MAPK antibody. The experiment was replicated three times