Fig. 1.

Expression profiling and copy number determination of the endogenous SAPK9 gene in 11 selected rice genotypes. Real-time PCR analysis showing SAPK9 gene expression in (a) vegetative stage and (b) reproductive stage (grain filling) in rice plants growing under the condition of before stress (BS), after stress (AS) and after recovery (AR). For internal reference, rice polyubiquitin1 (OsUbi1) gene was used. Error bars represent the mean ± SD of triplicate measurements. Student’s t-test was performed to find out statistically significant differences (* P < 0.05, **P < 0.01). c Southern hybridization blot depicting the presence of one copy of the endogenous SAPK9 gene in all the rice genotypes examined. Lanes 1-11 represents O. rufipogon, O. nivara, Nagina22, Manipuri, Vandana, Swarna, IR20, IR36, IR64, IR72 and HRC300 genotypes, respectively. d Multiple amino acid sequence alignment of subclass III SnRK2 family members including SAPK9 protein for the prediction of secondary structures following the PROSITE ExPASy bioinformatics tool