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. 2016 Jul 13;16:158. doi: 10.1186/s12870-016-0845-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Analysis of malondialdehyde (MDA) content and reactive oxygen species (ROS) activity in SAPK9 OE and RNAi plants. a Estimation of MDA content in leaf tissues of transgenic and NT plants before (Normal) and after drought stress (Drought). Error bars represent the mean ± SD of triplicate measurements. Student’s t-test was performed to find out statistically significant differences (**P < 0.01). b Monitoring ROS activity, as revealed by evaluation of H₂O₂ content in leaf tissues of transgenic and NT plants through visualization upon staining with DAB under normal and drought conditions. c Accumulation of O^2- ions in leaf tissues was visualized by staining with NBT in transgenic and NT plants under normal and drought stress conditions. Results were recorded from three independent experimental sets, and the result from one set is documented here