Table 2.

Primers used in this study.

Primers	Primer sequences(5′–3′)	Amplicon size	Restriction sites
cj0371-F1	GTGCAAGCTTCGCAAAAAACAAAAAATT	1.0 kp	Hind III
cj0371-R1	GCGCGAATTCACTTCTTGCGCTGCAGCA		EcoR I
cmr-F	GATCTGCAGTGGAGCGGACAACGAGTAAA	1.1 kp	Pst I
cmr-R	GATCTGCAGTCAGTGCGACAAACTGGGATT		Pst I
cj0371-F2	ATAGGATCCATGAAAAAAATCAAAAAA	606 bp	BamH I
cj0371-R2	GTAGAGCTCTTAAGAGCCAAAAGAAGA		Sac I
cj0371-F3	CGCGGATCCATGAAAAAAATCAAAAAA	603 bp	BamH I
cj0371-R3	ATCCGGATCCAGAGCCAAAAGAAGAAC		BamH I
PmetK-F	GCGTCTAGATAATTTCCGCTTGAAAGAGCA	592 bp	Xba I
PmetK-R	CGCGGATCCTCCTTTCATTTAAAATGAACC		BamH I