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. 2016 Jul 13;47:70. doi: 10.1186/s13567-016-0354-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Standardization of SYBR green-based RT-qPCR for ORF 1a gene. A Standard curve, B amplification plot and C melt curve analysis over a linear dynamic range from 1 log₁₀ to 7 log₁₀ copies/reaction.