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. 2016 Jul 13;47:70. doi: 10.1186/s13567-016-0354-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantification and identification of IBV B1648 and M41 infected total and KUL01 ⁺ PBMC. IBV B1648 and M41, and PBS (mock) inoculated blood samples were collected at 0, 2, 4, 6, 8, 10 and 12 dpi; 200 000 mononuclear cells were cytospinned and immunostainings were performed. B1648⁺ infected PBMC were observed at 2, 4 and 6 dpi and KUL01⁺B1648⁺ infected cells were observed at 4 and 6 dpi.