Effect of kisspeptin or kisspeptin antagonist injection into the posterodorsal medial amygdala (MePD) on LH secretion in male rats. Representative LH profiles demonstrating the effect of bilateral intra-MePD administration (↓) of vehicle (artificial cerebrospinal fluid; aCSF) (A), kisspeptin-10 (kp-10, 0.1 nmol) (B), kp-10 (1 nmol) (C), or kisspeptin receptor antagonist (P-234, 5 nmol) (D) on LH secretion. Direct administration of kp-10 into the amygdala dose-dependently increases LH secretion (B, C) shown by increased area under curve (AUC) of the LH profile (E). Kisspeptin antagonist had no effect on LH secretion (D, E). *p < 0.05, compared with vehicle. #p < 0.05, compared with dose of 0.1 nmol kp-10 at same time point; n = 4–6 per group. Data presesented as mean and SEM.