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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: Cancer Res. 2016 May 23;76(12):3484–3495. doi: 10.1158/0008-5472.CAN-15-2663

Figure 1. Mice with specific deletion of integrin beta3 in myeloid cells promoted tumor growth with increased M2 TAM infiltration.

Figure 1

(A) Growth curve of subcutaneous injected B16F10-Luc cells (1×106) in 8-week-old male WT (LysM-Cre+/−, Itgb3f/+) and β3KOM (LysM-Cre+/−, Itgb3f/−) mice (n=8). (B) Tumor weight. (C) Growth curve of MFP injected PyMT-BO1-GFP-Luc cells (1×105) in 8-week-old female WT and β3KOM mice (n=6). (D) Tumor weight. (E and F) FACS staining was performed on cells from day 11 tumor tissue. TAMs were gated on CD45+, CD11b+, Ly6G−, Ly6C−, F4/80+ population. In this population, M1 TAMs were gated as MHCIIhi/CD206low cells, and M2 TAMs were gated as MHCIIlow/CD206hi cells. (G) M2 TAM markers CD163, Fizz1, Arg1, and Ym1 mRNA expression in CD11b+ cells isolated from day 11 tumor tissue (n=3). Data represent mean ± SEM. *P < 0.05; **P < 0.01.