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Sequence alignment of cTnC and ssTnC, highlighted to visualize the changes in secondary structure after equilibration at each temperature and energy minimization. The most salient change is in helix C, and it is likely due to the temperature-dependent effect of the P55Q, I61V, and E75D substitutions on the stability of helix C and its interaction with helix D. To see this figure in color, go online.
