Table 3.

Sensitivity and specificity of HtLAMP-Pk compared with PCR on symptomatic, microscopy positive clinical DBS samples

	Sensitivity (N = 26)	Specificity (N = 20)	Specificity, excluding Plasmodium vivax (N = 5)
HtLAMP-Pk	96% (25), 95% CI 80–99	30% (6), 95% CI 12–54	100% (5) 95% CI 48–100
HtLAMP-Pk Iseki	81% (21), 95% CI 61–93	100% (20), 95% CI 83–100	–
HtLAMP-Pv	42% (11), 95% CI 23–63	30% (6), 95% CI 12–54	–

CI = confidence interval; DBS = dried blood spot; HtLAMP = high-throughput loop-mediated isothermal amplification; LAMP = loop-mediated isothermal amplification; PCR = polymerase chain reaction. The diagnostic accuracy of HtLAMP using three different LAMP primers was compared with PCR on DBS samples that were PCR positive for Plasmodium knowlesi (N = 26) and PCR negative for P. knowlesi (N = 20).