Table 1.
Characteristics of nine commercial CHIK IgM detection assays
| Manufacturer | Assay name and format | No. of samples per kit | Sample volume (μL) | Estimated time to test 20 samples (hours) | Storage conditions (°C) | Evaluated by laboratory |
|---|---|---|---|---|---|---|
| Microplate MAC-ELISA | ||||||
| Abcam (Cambridge, UK) | Anti-CHIKV IgM human ELISA | 91 | 10 | 4 | 2–8 | CARPHA, CDC |
| CTK Biotech (San Diego, CA) | Recombilisa CHIK IgM Test | 91 | 10 | 2 | 2–8 | CDC |
| Euroimmun (Luebeck, Germany) | Anti-CHIKV ELISA (IgM) | 93 | 2 | 3.5 | 2–8 | CARPHA, CDC, and NML |
| Genway (San Diego, CA) | CHIKV IgM μ-capture ELISA | 91 | 10 | 4 | 2–8 | CDC |
| InBios (Seattle, WA) | CHIKjj Detect MAC-ELISA | 92 | 4 | 3.5 | Most 2–8; antigen −20 to −80 | CARPHA, CDC |
| SD Standard Diagnostics (Yongin-si, Gyeonggi-do, Republic of Korea) | CHIKa IgM ELISA | 91 | 10 | 2 | 2–8 | CDC |
| Rapid test | ||||||
| CTK Biotech | On-site CHIK IgM Combo Rapid test | 30 | 30 | 0.5 | 2–30 | CDC |
| SD Diagnostics | SD BIOLINE Chikungunya IgM | 25 | 50 | 0.5 | 1–30 | CDC |
| Indirect immunofluorescence assay | ||||||
| Euroimmun | Anti-CHIKV IIFT (IgM)* | 50 | ∼15 | 3 | 2–8 | CARPHA, CDC |
CARPHA = Caribbean Public Health Agency; CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IIFT = indirect immunofluorescent test; MAC-ELISA = IgM antibody-capture enzyme-linked immunosorbent assay; NML = the Public Health Agency of Canada National Microbiology Laboratory.
*
Immunosorb IgG depletion sample buffer purchased separately.