Table 4.
Summary results of nine CHIKV IgM detection assays evaluated at CDC
| Test | Kit result | CDC CHIKV IgM+* | CDC CHIKV IgM−† | Total |
|---|---|---|---|---|
| CTK Recombilisa CHIK IgM Test | + | 2 | 0 | 2 |
| − | 14 | 4 | 18 | |
| Total | 16 | 4 | 20 | |
| Genway CHIKV IgM μ-capture ELISA | + | 0 | 0 | 0 |
| − | 27 | 16 | 43 | |
| Total | 27 | 16 | 43 | |
| SD Diagnostics CHIKa IgM ELISA | + | 12 | 2 | 14 |
| − | 19 | 11 | 30 | |
| Total | 31 | 13 | 44 | |
| Abcam Anti-CHIKV IgM human ELISA (30 minutes 25°C incubation), lot no. GR166537-2 | + | 44 | 2 | 46 |
| − | 3 | 37 | 40 | |
| EQ | 1 | 3 | 4 | |
| Total | 48 | 42 | 90 | |
| Abcam ELISA (ON 4°C incubation), lot no. GR166537-2 | + | 47 | 1 | 48 |
| − | 1 | 40 | 41 | |
| EQ | 0 | 1 | 1 | |
| Total | 48 | 42 | 90 | |
| Abcam ELISA (30 minutes 25°C incubation), lot no. GR232915-1 | + | 36 | 1 | 37 |
| − | 0 | 32 | 32 | |
| EQ | 0 | 1 | 1 | |
| Total | 36 | 34 | 70 | |
| Euroimmun Anti-CHIKV ELISA (IgM) | + | 51 | 1 | 52 |
| − | 1 | 38 | 39 | |
| EQ | 0 | 1 | 1 | |
| Total | 52 | 40 | 92 | |
| InBios CHIKjj Detect MAC-ELISA | + | 36 | 0 | 36 |
| − | 0 | 35 | 35 | |
| Total | 36 | 35 | 71 | |
| CTK On-site CHIK IgM Combo RT | + | 3 | 0 | 3 |
| − | 20 | 4 | 24 | |
| Total | 23 | 4 | 27 | |
| SD BIOLINE Chikungunya IgM | + | 0 | 0 | 0 |
| − | 24 | 7 | 31 | |
| Total | 24 | 7 | 31 | |
| Euroimmun Anti-CHIKV IIFT (IgM) | + | 34 | 0 | 34 |
| − | 3 | 35 | 38 | |
| EQ | 0 | 3 | 3 | |
| Total | 37 | 38 | 75 |
CARPHA = Caribbean Public Health Agency; CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; EQ = equivocal; MAC-ELISA = IgM antibody capture enzyme-linked immunoassay; IIFT = indirect immunofluorescence test; ON = overnight; RT = rapid test.
*
Includes Mayaro and o'nyong-nyong viruses IgM PC serum.
†
Includes Venezuelan equine encephalitis and North American eastern equine encephalitis viruses IgM PC serum.