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Maximum likelihood tree of 91 DENV-1 E gene sequences (1,485 bp) including 24 Costa Rican strains (black rectangle), 20 obtained from this study (bold font), and four obtained from GenBank (italic font). Significant bootstrap values (≥ 70) are indicated at the respecting nodes. The sequences were named according to reference number/country/year of collection or isolation. Major clades and subclades comprising Costa Rican strains are indicated with numbers and letters, respectively.
