Figure 1.

Identification of amyloidogenic clone by Multi‐parameter flow cytometry. Gating strategy for the identification of light chain‐restricted plasma cells (PCs) (A–C) and B cells (D–F). PCs (green, B) were identified as CD38− and CD138‐positive cells among leukocytes (blue, A). PCs were anticipated as monoclonal when light chain restriction was observed. Figure C depicts a λ light chain‐restricted PC population (green), whereas κ and λ expression was noted on polyclonal B cells (red). B cells (green, E) were detected as a CD19‐positive population among leukocytes (blue, D). Light chain restriction on B cells was identified with the κ/λ gate (F). CD, cluster of differentiation; FSC‐A, forward scatter area; SSC‐A, side scatter area.