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. 2016 Jul 14;11(7):e0158969. doi: 10.1371/journal.pone.0158969

Fig 1. α-toxin induces AREG shedding and IL-8 production from HVECs.

Fig 1

HVECs were exposed to α-toxin for 6 h and then processed for toxicity via MTT assay or IL-8 secretion and AREG shedding via ELISA. Where inhibitors were used, they were applied to HVECs 30 min prior to addition of α-toxin. (A) IL-8 secretion from and (B) viability of HVECs exposed to various doses of α-toxin. For both curves, the asterisks indicate doses showing significant differences from 0 (p < 0.0001). (C) AREG shedding and (D) IL-8 secretion in response to α-toxin is dampened in the presence of TAPI-1 and AG1478. White bars indicate media alone, black bars represent α-toxin treatment at 1 μg/ml. Asterisks indicate significant differences from α-toxin (AT) alone (p < 0.0001). (E) AREG shedding and (F) IL-8 secretion in response to α-toxin are dampened in ADAM10 (A10) and ADAM17 (A17) KD cell lines. White bars indicate media alone on negative control (NC) siRNA cells, black bars represent α-toxin treatment at 1 μg/ml. Asterisks indicate significant differences from α-toxin treated NC cells (p < 0.0001).