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. 2016 Jul 14;11(7):e0158969. doi: 10.1371/journal.pone.0158969

Fig 2. IL-8 production from PVM in response to TSST-1 and α-toxin is dependent on EGFR signaling.

Fig 2

PVM explants were exposed to TSST-1 and/or α-toxin for 6 h and then processed for IL-8 production via ELISA. Where inhibitors were used, they were applied to explants 30 minutes prior to addition of toxin(s). IL-8 is produced in response to both (A) TSST-1 and (B) α-toxin in a dose-dependent manner. For both curves, the asterisks indicate doses showing significant differences from 0 (p < 0.0004). (C) IL-8 production in response to high doses of both TSST-1 (20 μg/explant) and α-toxin (AT) (2 μg/explant) is completely abrogated in the presence of AG1478 (AG– 40 μg/explant), but the dextrin vehicle (Dex– 10 μl of 15%) alone has no affect. Checkered bars represent TSST-1 treatment and striped bars represent AT treatment. Asterisks indicate significant differences from media alone, while crosses indicate significant differences from toxin alone (p < 0.0003). (D) Low doses of TSST-1 (5 μg/explant) and AT (25 ng/explant) have an additive effect on IL-8 production that is reduced to basal levels in the presence of AG1478 (4 μg/explant) with no dextrin vehicle effect (10 μl of 15%). White bars indicate media alone, checkered bars represent TSST-1 treatment, striped bars represent AT treatment, black bars represent TSST-1 + AT treatment. Asterisk indicates significant difference from media, TSST-1 and AT alone (p < 0.03), while cross indicates significant difference from TSST-1 + AT (p < 0.0009).