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. 2016 Jul 14;11(7):e0158966. doi: 10.1371/journal.pone.0158966

Fig 7. Transactivation activities of BrABI5a and BrABI5b.

Fig 7

(A) Yeast one-hybrid analysis of BrABI5a and BrABI5b. Yeast lines yWAM2 expressing the indicated plasmids were grown on synthetic complete medium without Leu and Trp (SC-LW; left) and on synthetic complete medium without Leu, Trp, and His (SC-HLW; right). Yeast cells were incubated until the optical density at 600 nm reached 0.5 and then diluted 2-fold (×2), 10-fold (×10), 50-fold (×50), or 250-fold (×250) and used for assays. (B) Transactivation activity BrABI5a or BrABI5b in Arabidopsis leaf mesophyll protoplasts. Transactivation experiments were performed using protoplasts prepared from Col-0 leaves. Transfected cells were cultured for 16 h without or with 5μM ABA, and relative LUC activity was assayed according to the Dual-Luciferase Reporter Assay Protocol provided by Promega. The empty vector control was also included as a negative control. The values shown are average fLUC (firefly, Photinus pyralis, LUC) activities normalized to rLUC (sea pansy, Renilla reniformis, LUC) activities. BrABI5aΔbZIP and BrABI5bΔbZIP are forms of BrABI5a and BrABI5b that carries a deletion of the intact C-terminal bZIP region respectively.