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. 2016 Jul 14;11(7):e0158966. doi: 10.1371/journal.pone.0158966

Fig 8. Heterogeneous expression of BrABI5a and BrABI5b reverse the insensitivity of Arabidopsis abi5-1 to ABA during seed germination.

Fig 8

(A, B) Sensitivity of seed germination to ABA. The seeds of Ws-2, abi5-1, and transgenic abi5-1 lines carrying Myc-tagged BrABI5a or BrABI5b (abi5-1::Myc-BrABI5a or abi5-1::Myc-BrABI5b) were germinated on MS medium (A) and MS medium supplemented with 3μM ABA (B) for the indicated days. The emergence rate of green cotyledons (C) and radicle (D) from Ws-2, abi5-1 and abi5-1::Myc-BrABI5a or abi5-1::Myc-BrABI5b transgenic seeds plated on MS supplemented with ABA. Approximately 150 seeds were used in each experiment. Error bars represent SD (seed number > 100). (E) Immunoblots of Myc-BrABI5a or Myc-BrABI5b protein levels in the transgenic abi5-1 lines (abi5-1::Myc-BrABI5a or abi5-1::Myc-BrABI5b). N, transgenic abi5-1 lines carrying the empty Myc-tagged vector; CBB (Coomassie Brilliant Blue) R250-stained RLS (Rubisco large subunit) served as a loading control.