Skip to main content
. 2016 Jul 14;11(7):e0159275. doi: 10.1371/journal.pone.0159275

Fig 5. Spry2 suppresses ERK1/2 phosphorylation in human lens epithelial cells.

Fig 5

(A-B) Cultured human lens epithelial cells were transfected with Spry2 siRNA or Spry2 plasmid and treated with or without TGFβ for 48h. Untransfected cells, cells transfected with scrambled siRNA or empty vector were used as controls. Proteins were extracted and probed for pERK1/2 and total ERK1/2. β-actin was used as a loading control. (C-D) Quantification of the pERK1/2 and total ERK1/2 expression levels in A and B, respectively. Fold change relative to the level of untransfected groups is displayed. **P<0.01, ***P<0.001, n = 3.