Skip to main content
. 2016 Jul 14;11(7):e0159275. doi: 10.1371/journal.pone.0159275

Fig 6. EMT induced by Spry2 downregulation was inhibited by Smad and ERK1/2 specific inhibitors.

Fig 6

(A) Cultured human lens epithelial cells were transfected with scrambled siRNA or Spry2 siRNA and treated with Smad inhibitor SB431542 (10.0μM) or ERK1/2 inhibitor U1026 (10.0μM) or both for 24h. Proteins were extracted and probed for pSmad, total Smad, pERK1/2, total ERK1/2, α-SMA and Fn. β-actin was used as a loading control. (B-D) Quantification of the protein expression levels in A. Fold change relative to the level of the scrambled siRNA transfected group is displayed. *P<0.05, **P<0.01, ***P<0.001, NS: not significant, n = 3.