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. 2016 Jul 14;12(7):e1005759. doi: 10.1371/journal.ppat.1005759

Fig 3. Within-tick Bb diversity in single infected ticks.

Fig 3

(a) The minor allele frequency (MAF) spectrum of a representative “singly” infected tick sample. The Bb population infecting Bbcap22 (600 X coverage) harbors few variants with MAF < 5%, likely accrued via in situ evolution. Histogram bars represent the count of genomic sites with a minor allele frequency that falls within the MAF frequency bin on the x-axis. Note the differing y-axes for (a) and (c, d, and e). (b) MAF spectrum of a “multiply” infected tick sample, Bbcap5 (109 X coverage). The Bb population infecting Bbcap5 harbors a high number of intermediate frequency minor alleles, a level of diversity likely preexisting within a diverse inoculum. (c) MAF spectrum of a “multiply” infected tick sample, Bbcap17 (221 X coverage). (d) MAF spectrum of a “multiply” infected tick sample, Bbcap31 (309 X coverage).