Fig 3. Evaluation of microarray quality in terms of array design and printing markers.

(A) Representative image of a microarray slide with 14 identical subarrays. Each subarray (n = 14) is divided into 3 segments separated by dispensed rows of reference marker replicate spots (n = 4). (B) Blank replicate spots (n = 5) detected in the marked area. No signal intensity was obtained from the dispensed blank replicate spots after scanning the microarray using a wavelength of 633 nm, while positive spots at 543 nm (Cadaverine) confirmed that spotting had occurred.