Fig 5. Limit of detection.

Boxplots for 5 antibody intensities over all the analyzed samples. Each data point represents one sample. A cut-off limit was established based on the mean negative control signal (PBS) across all the samples plus 2 standard deviations. Each analyte, from which the mean signal intensities were found to be below the LOD in > 70% of samples was removed from the data (e.g. FASN (3) and MAKT (2)). The red line corresponds to the cut-off limit.