Table 3. Evaluation of different normalization methods.

To this end, microarray data was used to assess the No of statistically significant scFvs (n_total = 195) when comparing day 1 vs. day 2, using a cut-off value of either q <0.05 or q <0.01 as well as q < 0.05 plus FC > 1.5.

Normalization method	All subjects except QC-ref samples, at q < 0.05	Only QC-ref samples, at q < 0.05	Only QC-ref samples, at q < 0.01	Only QC-ref samples, at q < 0.05 plus FC > 1.5
Un-normalized log2 transformed data	163	167	158	43
Subtract by group mean + semi-global	0	61	37	6
ComBat + semi-global	0	59	35	5
Global VSN + Combat	0	46	29	4
Global LOESS + Combat	0	47	26	6