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. 2016 Jul 14;12(7):e1005758. doi: 10.1371/journal.ppat.1005758

Fig 3. Analysis of TcdA and TcdB production.

Fig 3

a, c, e, Western immunoblot using TcdA-specific and TcdB-specific antibodies with precipitated supernatant from the strains indicated. CD37 (non-toxigenic), V = vector control, R+ = cdtR complemented. Arrows indicate the 308 kDa TcdA and 270 kDa TcdB proteins. Supernatants were collected at 12, 24, 48 and 72 hours post inoculation and assayed by doubling dilution cytotoxicity assays. b, The panel of M7404 strains were assayed using HT29 cells and Vero cells. d, R20291 panel of strains assayed using HT29 cells and Vero cells. Data represent the mean ± SEM (n = 3–5). *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001.