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RNA was isolated from strains for analysis of (a) tcdA, (b) tcdB, (c) cdtA, (d) tcdR, (e) tcdC and (f) sigD expression. Levels of gene expression were normalised to rpoA. Data represent the mean normalised gene expression ± SEM from five independent biological replicates. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001.
