|
Strain
|
|
|
|
E. coli
|
|
|
| DH5α |
F– Φ80dlacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rK
–, mK
+) deoR thi-1 supE44 gyrA96 relA1
|
Life Technologies |
| Top10 |
F–mcrA Δ(mrr
-
hsdRMS
-
mcrBC) Φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 λ–
|
Life Technologies |
| MM294 |
F–, endA1, hsdR17(rK
–mK
+), supE44, thi-1, recA
+
|
[50] |
|
B. subtilis
|
|
|
| BS34A |
B. subtilis donor that carries a single copy of Tn916 on its chromosome, TcR
|
[51] |
| JIR6342 |
BS34A carrying cdtR TargeTron plasmid, pJIR4153 |
This study |
| JIR6345 |
BS34A carrying cdtR complementation plasmid, pJIR4218 |
This study |
| JIR6346 |
BS34A carrying shuttle plasmid, pDLL24 |
This study |
|
C. difficile
|
|
|
| M7404 |
Canadian BI/NAP1/027 isolate |
[6] |
| R20291 |
UK BI/NAP1/027 isolate |
[39] |
| CD37 |
Non-toxigenic C. difficile isolate |
[52] |
| JGS6133 |
Porcine ribotype 078 isolate |
[12] |
| DLL3094 |
M7404 (pDLL24) |
This study |
|
cdtR1 (or JIR8707) |
M7404 cdtR::TargeTron (mutant 1), LnR
|
This study |
|
cdtR2 (or JIR8708) |
M7404 cdtR::TargeTron (mutant 2), LnR
|
This study |
| JIR8739 |
JIR8707 (pDLL24), LnR, TmR
|
This study |
| JIR8740 |
JIR8707 (pJIR4218), LnR, TmR
|
This study |
| JIR8741 |
JIR8708 (pDLL24), LnR, TmR
|
This study |
| JIR8742 |
JIR8708 (pJIR4218), LnR, TmR
|
This study |
| JIR8729 |
R20291 cdtR::TargeTron, LnR
|
This study |
| JIR8745 |
JIR8729 (pDLL24), LnR, TmR
|
This study |
| JIR8746 |
JIR8729 (pJIR4218), LnR, TmR
|
This study |
| JIR8747 |
R20291 (pDLL24), TmR
|
This study |
| JIR8733 |
JGS6133 (pDLL24), TmR
|
This study |
| JIR8735 |
JGS6133 (pJIR4218) |
This study |
|
Plasmid
|
|
|
| pDLL4 |
C. difficile shuttle vector allows plasmid to be mobilised by Tn916, TmR
|
[12] |
| pDLL24 |
pDLL4 carrying lacZα, CmR
|
This study |
| pJIR4218 |
pDLL24 carrying cdtR and its promoter, CmR
|
This study |
| pDLL45 |
pMTL9361 derivative with HindIII and BsrGI sites removed from rep; TmR
|
This study |
| pDLL55 |
Derivative of pDLL45 carrying lacZα; TmR
|
This study |
| pJIR4135 |
Group II intron of pDLL45 retargeted to insert between codons 288/289 of the cdtR gene, CmR
|
This study |
| pJIR4153 |
pDLL55 (StuI/HindIII)Ω pJIR4135 group II intron (StuI/HindIII) |
This study |
