Fig 7. Pf-SSBMDCC does not influence the activity of Srs2.
(A) Change in fluorescence upon mixing varying concentrations of Pf-SSBMDCC (100 or 200 nM) with buffer in the presence or absence of m13ssDNA (3 μM nucleotides). (B) Rad51 filament clearing by Srs2 was measured in the presence of increasing concentrations of Pf-SSBMDCC. Preformed Rad51 filaments were rapidly mixed with varying amounts of Pf-SSBMDCC (75, 100, 125 or 150 nM) and Srs2 (100 nm) and the change in fluorescence was measured over time. Data were collected over a split time period with 5000 points each assigned to the first 10 sec and remaining 50 sec, respectively. An average of 10 independent traces is shown. (C) The normalized change in fluorescence at time = 10 sec was subtracted from time = 0.01 sec and the Δfluorescence@10sec values plotted as a function of [Pf-SSBMDCC]. No significant change in fluorescence is observed. The mean values and standard errors from three independent experiments are shown.