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. 2016 Jul 14;10(7):e0004799. doi: 10.1371/journal.pntd.0004799

Fig 3. Exogenous DIII and stem fragments inhibit the infection of cells by ANDV.

Fig 3

(A, B) Infection of Vero E6 cells by ANDV in the presence of exogenous DIII (A) or stem peptides (B). 100% infection was defined as the condition in the absence of Gc-derived fragments (Mock). (C, D) Transduction of Vero E6 cells by SIV vectors pseudotyped with VSV-G (C) or by SIV vectors pseudotyped with ANDV Gn/Gc glycoproteins (GPC; D) in the presence of exogenous DIII or stem peptides. Transduction was assessed by GFP reporter gene expression using flow cytometry, and 100% transduction defined as transduction in the absence of Gc-derived fragments (Mock). (E) Cytotoxicity analysis of Gc DIII proteins and stem peptides on Vero E6 cells. (F) ANDV inhibition by the R2 stem peptide through pre-incubation (pre-R2) or co-incubation (co-R2). For pre-incubation, ANDV was first incubated for 1 h with different concentrations of R2, and subsequently the mixture was incubated for 1 h with cells. For co-incubation, the virus and the R2 peptide were simultaneously added to the cells and incubated for 1 h. Infection was quantified in both cases by flow cytometry 16 h post-infection.