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. 2016 Jul 15;27(14):2186–2197. doi: 10.1091/mbc.E16-02-0086

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© 2016 Forteza et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Apical and junction markers and intestinal epithelium ultrastructure in Prkci^flox/flox Vil-CRE± (KO) mice. (A) Frozen sections of villus enterocytes from KO and control littermates (lm) were processed with the following antibodies: alkaline phosphatase (iAP), active (pT567) ezrin, ezrin, ZO-1, and human homologue of Par3. Ezrin inset, cross sections of the crypts displaying normal apical ezrin levels. Bars, 10 μm (iAP, pT567ezrin), 45 μm (ezrin), 25 μm (ZO-1, Par3). (B–E) Electron microscopy images of villus enterocytes from control (B, D) or KO (C, E) animals. Bars, 10 μm (B, C), 0.5 μm (D, E).