FIGURE 3:

The 611–950 segment of Hsl1 contains two separable septin-association elements. (A) Right, diagram of the indicated internal fragments, each fused to the same C-terminal fragment (and eGFP), that were examined. Left, plasmids producing the indicated constructs (pGF-IVL536 and pGF-IVL601 through pGF-IVL611), expressed and visualized as in Figure 1, A and B. Scale bar, 2 μm. (B) Right, diagram of the same internal fragments as in A, each fused to eGFP only, that were examined. Left, plasmids producing the indicated constructs (pGF-IVL612 through pGF-IVL623), expressed and visualized as in Figure 1, A and B. Dotted white line, cell periphery; scale bar, 2 μm. For clarity, only one or a few representative cells are shown for each construct, and the dotted white line is omitted for constructs that exhibited significant PM fluorescence.