FIGURE 4:

A conserved sequence element within the 611–950 segment of Hsl1 makes a major contribution to its bud neck localization. Top, diagram of the indicated internal deletions and the Hsl1(T665A L666A N668A S669A S671A K672A R673A S674A L675A Y676A S677A S680A I681A S682A K683A R684A S685A N687A L688A) allele that were examined. Bottom, plasmids producing the indicated deletions and substitution mutant (pGF-IVL666, pGF-IVL665, pGF-IVL710, pGF-IVL709, pGF-IVL711, and pGF-IVL816), expressed and visualized as in Figure 1, A and B. Arrowheads, GFP signal in PM-associated puncta; dotted white line, cell periphery; scale bar, 2 μm. For clarity, only one or a few representative cells are shown for each construct, and the dotted white line is omitted for constructs that exhibited significant PM fluorescence.