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. 2016 Jul 15;27(14):2234–2244. doi: 10.1091/mbc.E15-10-0734

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© 2016 Sato et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Normal growth and virulence of transgenic asexual blood-stage parasites. (A) Western blot analysis of FLAG-tagged proteins in schizonts. Samples were probed with an anti-FLAG antibody, with anti-tubulin antibodies used as control. (B) Immunofluorescence micrographs of schizonts stained with anti-FLAG (green), anti-MSP1 (red) antibodies, and 4′,6-diamidino-2-phenylindole (DAPI; blue). Bars, 2 μm. (C) Parasitemia development after intravenous injection of 1000 infected erythrocytes. Growth curves of asexual blood-stage parasites are similar in all transgenic parasite lines (n = 6). (D) Kaplan–Meier analysis of mice that exhibit signature symptoms of ECM. All infected animals developed ECM symptoms, except for one profilin^AMA1-infected mouse. Analysis by Kruskal–Wallis and Mantel–Cox tests indicated that differences are nonsignificant.