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. 2016 Jul 15;27(14):2234–2244. doi: 10.1091/mbc.E15-10-0734

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© 2016 Sato et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Normal ookinete motility and Anopheles infections in transgenic parasite lines. (A) Western blot analysis of FLAG-tagged proteins in ookinetes. Protein extracts of 100,000 purified ookinetes were separated by SDS–PAGE. Samples were probed against an anti-FLAG antibody and anti-tubulin antibody as control. (B) Immunofluorescence micrographs of ookinetes stained with anti-FLAG antibody and DAPI. Differential interference contrast images are included to display ookinetes (bars, 5 μm). (C) Time-lapse micrographs of representative ookinete at 0, 300, and 600 s. The line reflects ookinete motility in Matrigel (bars, 10 μm). (D) Representative velocities of transgenic and WT ookinetes over a recording period of 500 s. Each frame corresponds to 5 s. (E) Average speed (μm/s) was quantified from time-lapse movies (n = 20 each). The results represent mean values (± SD) of three independent experiments. Differences are nonsignificant (Mann–Whitney and Kruskal–Wallis tests). (F) Oocyst numbers in infected mosquitoes. The results represent mean values (± SD) of five independent natural feeding experiments.