Figure 3. IL-36γ mRNA is induced in murine PMs and human AMs in vitro in response to Gram-positive and -negative bacteria.

(A) WT PMs were treated with live or HK Sp (MOI 10:1). Cell lysates were collected at 6 and 18 h and assessed for IL-36γ mRNA by RT-PCR. Data are means ± sem of 3 wells/group (10⁶ cells/well). *P < 0.05 vs. control for each time point. (B) WT and MyD88^−/− PMs were treated with LPS (100 ng) or with live or HK Kp (MOI 10:1). Cell lysates were collected at 18 h and assessed for IL-36γ mRNA by RT-PCR. *P < 0.05 vs. control. (C) Human AMs were treated with live or HK Kp (MOI 10:1). Cell lysates were collected at 4 and 18 h and assessed for human IL-36γ mRNA by RT-PCR. Data are means ± sem of 4 wells/group (10⁶ cells/well). *P < 0.05 vs. control for each time point.