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. 2016 Apr 5;100(2):381–389. doi: 10.1189/jlb.3A1015-474R

Figure 1. FBs promote the IL-23–Th17 axis during irradiation.

Figure 1.

IR suppresses IL-23. DCs were irradiated before addition of LPS/IFN-γ and IL-23 release measured by ELISA. (A) IL-23 suppression was dependent on the dose of IR (a representative donor from 3). (B) A representative experiment at 6 Gy. (C) A summary of 18 donors. (D) FBs rescue IL-23 secretion by irradiated DCs. Coculture of irradiated FBs with irradiated DCs up-regulated IL-23 secretion (summary of 8 donors). (E) Fold change of IL-23 secretion by irradiated DC/FB cocultures at 0 and 6 Gy. (F) Primary dermal FBs demonstrate similar IL-23-enhancing activity to the BJ6 cell line (data from a representative donor or 5). (G) FBs permit irradiated DCs to promote Th17 responses. IL-17A secretion by naïve CD4+ T cells activated with anti-CD3/anti-CD28 in the presence of supernatants of the indicated DC/FB cultures. T cells were stimulated for 5 d and IL-17A secretion determined after restimulation (collective data from 3 donors). Error bars indicate sd of triplicate experiments. **0.01 > P ≥ 0.001, ***0.001 > P ≥ 0.0001, ****P < 0.0001; ns, not significant. Results are presented as means ± sd.