Figure 3. FBs are functionally resistant to IR.

Irradiation of FBs did not impair their ability to up-regulate IL-23 secretion from nonirradiated DC. (A) A representative donor. (B) Summary of 3 donors. (C) PGE₂ secretion (determined by ELISA) by FBs stimulated with rTNF-α and rIL-1β (1 ng/ml) is unaffected by IR; BJ6 cell line. (D) Representative results for 1 of 2 primary FB donors. (E) IR (6 Gy) does not affect FB viability up to 24 h after irradiation, as assessed by annexin V/PI staining; representative data from 3 experiments. ***0.001 > P ≥ 0.0001. Results are presented as means ± sd.