TABLE 1.
Cytokine | % Remaining secretion | sd | P |
---|---|---|---|
IL-23 | 43 | 11 | <0.0001 |
IL-12 | 89 | 16.3 | 0.0005 |
IL-27 | 100.9 | 36.6 | ns |
IL-1β | 93.5 | 34.4 | ns |
TNF-α | 111.8 | 29.6 | ns |
IL-6 | 174.1 | 114.4 | ns |
IL-10 | 95.9 | 6.2 | ns |
DCs were irradiated (6 Gy) or nonirradiated before activation with LPS/IFN-γ. Supernatants were collected following 24 h incubation, and secreted IL-23, IL-12, IL-27, IL-1β, TNF-α, IL-6, and IL-10 were measured by ELISA. All experiments were repeated at least 4 times in triplicate, and significance was assessed with 2-way ANOVA.