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. Author manuscript; available in PMC: 2017 Jan 7.
Published in final edited form as: Nature. 2016 Jul 7;535(7610):178–181. doi: 10.1038/nature18316

Extended Data Table 1.

Strains and plasmids used in this study. a,Bacterial strains used in this study. b, Plasmids used in this study. BGSC - Bacillus Genetic Stock Center (http://www.bgsc.org)

a
Name Genotype Source
JDW437 (wild-type 168) trpC2 Lab stock
JDW941 151 phi3T Ronald Yasbin
JDW942 168 thyA− thyB− Ronald Yasbin
JDW1297 PY79 mutSL::kan Lyle Simmons
JDW1543 168 ΔthyA This work
JDW1544 168 ΔthyA amyE::Pspank-thyP3 (head-on) spc This work
JDW1563 168 ΔthyA amyE::Pspank-thyP3 (co-directional) spc This work
JDW1711 168 ΔthyA amyE::Pspank-thyP3 (head-on) spc, lacA::Ppen-spoVG-lacZ This work
JDW1814 168 ΔthyA amyE::Pspank-thyP3 (head-on) spc, mutSL::kan This work
JDW1900 168 ΔthyA amyE::spc-Pspank-thyP3 (head-on) lacI This work
JDW1901 168 ΔthyA amyE::spc-Pspank-thyP3 (co-directional) lacI This work
JDW2054 168 ΔthyA amyE::Pspank-thyP3 (head-on) G+473→A+473 spc This work
JDW2057 168 ΔthyA amyE::Pspank-thyP3 (head-on) T−7→C−7 spc This work
JDW2185 168 ΔthyA amyE::Pspank-thyP3 (head-on) T+331→C+331 spc This work
JDW2190 168 ΔthyA amyE::Pspank-thyP3 (head-on) G+473→A+473 spc, lacA::Ppen-spoVG-lacZ This work
JDW2192 168 ΔthyA amyE::Pspank-thyP3 (head-on) T−7→C−7 spc, lacA::Ppen-spoVG-lacZ This work
JDW2266 168 ΔyqjH BGSC
JDW2284 168 ΔyxlJ BGSC
JDW2288 168 ΔrecA BGSC
JDW2491 168 thyA− thyB− amyE::Pspac(hy)-thyP3 Δ+102-145 deletion spc This work
JDW2492 168 thyA− thyB− amyE::Pspac(hy)-thyP3 TT+124 insertion spc This work
JDW2501 168 ΔadeC BGSC
JDW2529 168 ΔthyA amyE::Pspank-thyP3 (head-on) spc, ΔyqjH This work
JDW2530 168 ΔthyA amyE::Pspank-thyP3 (co-directional) spc, ΔyqjH This work
JDW2547 168 ΔthyA amyE::Pspank-thyP3 (head-on) spc, ΔadeC This work
JDW2548 168 ΔthyA amyE::Pspank-thyP3 (co-directional) spc, ΔadeC This work
JDW2598 168 ΔthyA amyE::Pspank-thyP3 (head-on) spc ΔrecA, This work
JDW2612 168 ΔthyA amyE::Pspank-thyP3 (co-directional) spc, ΔrecA This work
JDW2697 168 ΔthyA amyE::Pspank-thyP3 (head-on) spc, ΔyxlJ This work
JDW2746 168 ΔthyA amyE::Pspank-thyP3 (head-on) G+332→A+332 spc This work
JDW2747 168 ΔthyA amyE::Pspank-thyP3 (head-on) G+332→A+332 spc, lacA::Ppen-spoVG-lacZ This work
JDW2748 168 ΔthyA amyE::Pspank-thyP3 (head-on) +1G+335 spc This work
JDW2749 168 ΔthyA amyE::Pspank-thyP3 (head-on) +1G+335 spc, lacA::Ppen-spoVG-lacZ This work
b
Name Genotype Source
pDR90 amyE::Pspac(hy) amp spc David Rudner
pDR110 amyE::Pspank amp spc David Rudner
pJW299 pEX44/I-SceI site amp cat Lab stock
pJW331 pDR90/amyE::Pspac(hy)-thyP3(head-on) amp spc This work
pJW395 pJW299/ΔthyA I-SceI site amp cat This work
pJW396 pDR110/amyE::Pspank-thyP3 (head-on) amp spc This work
pJW397 pDR110/amyE::Pspank-thyP3 (co-directional) amp spc This work
pJW417 pEX44/lacA::Ppen-spoVG-lacZ amp cat This work
pJW430 pDR110/amyE::spc-Pspank-thyP3 (head-on) lacI amp This work
pJW431 pDR110/amyE::spc-Pspank-thyP3 (co-directional) lacI amp This work