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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Pflugers Arch. 2016 Mar 14;468(7):1161–1170. doi: 10.1007/s00424-016-1802-0

Figure 4. Sialytransferase ST6GalI directs UT-A3 sialylation and cell membrane expression.

Figure 4

UT-A3 membrane expression. HEK293 cells co-transfected UT-A3 without or with ST6GalI (ST6) or ST3GalIV (ST3) were processed for cell surface biotinylation. The total and the cell membrane UT-A3 proteins were examined by Western blot with FLAG antibody. UT-A3 protein sialylation was examined by SNA pull-down assay. Bar graph showed the cell membrane UT-A3 expression as a percent of control (** P<0.01, n=4).