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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Pflugers Arch. 2016 Mar 14;468(7):1161–1170. doi: 10.1007/s00424-016-1802-0

Figure 5. Activation of PKC pathway promotes UT-A3 sialylation.

Figure 5

A: UT-A3 sialylation by PDB. HEK 293 cells were transiently transfected with UT-A3 for 48h followed by PDBu treatment for 4h. Sialylated UT-A3 was pulled down by SNA and then examined by western blot. Con A lectin was used as a control. Bar graph showed the band densities of sialylated UT-A3 as a percent of control (0 PDBu) (** P<0.01, n=3). B: UT-A3 membrane expression. HEK293 cells were transiently transfected with UT-A3 alone or together with ST6GalI for 48h, and then treated with or without 2 μM chelerythrine (chel) for 2h. Cell surface proteins were biotinylated and analyzed by Western blot with FLAG antibody. C: Inhibition of ST6GalI-induced sialylation by PKC inhibitor. HEK293 cells transfected with UT-A3 alone or together with ST6GalI for 48h, then treated with chelerythrine for 2 h, sialylated UT-A3 was pulled down by SNA and then examined by western blot. B, C showed the representative western blots from three independent experiments.