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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Pflugers Arch. 2016 Mar 14;468(7):1161–1170. doi: 10.1007/s00424-016-1802-0

Figure 6. Effect of ST6GalI on UT-A3 protein degradation.

Figure 6

HEK293 cells transfected with UT-A3 alone or together with ST6GalI were treated with 100 μg/ml cycloheximide (CHX) for the indicated time. The cells were lysed with RIPA buffer and total UT-A3 protein levels were evaluated by Western blot with FLAG antibody. Bar graph showed the band densities of UT-A3 (including both 65- and 45-kDa) as a percent of control (0 h) (* P<0.05, ** P<0.01, n=3).