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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Pflugers Arch. 2016 Mar 14;468(7):1161–1170. doi: 10.1007/s00424-016-1802-0

Figure 7. Effect of ST6GalI on UT-A3 activity.

Figure 7

Oocytes were injected with cRNAs of UT-A3 (2 ng/cell) alone or plus ST6GalI (2 ng/cell). After 3 days, rea transport activity was measured by 14C-labeled urea flux (n=6 oocyte/time point; mean ± SD). UT-A3 protein expression on the cell membrane was examined by biotinylation and sialylated UT-A3 was pulled down by SNA, followed by Western blot with a UT-A1 NH2-terminal antibody (experiments n=3).