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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Membr Biol. 2016 Apr 2;249(4):503–511. doi: 10.1007/s00232-016-9892-3

Fig. 1. DRAQ5™-mediated inhibition of LtxA internalization in Jn.9 cells.

Fig. 1

(A) TR-LtxA (red) was incubated with K562 cells expressing CFP- and YFP-labeled LFA-1 (green). After 30 mins, TR-LtxA was found to be colocalized with lysosomes (blue). (B) K562 cells were first pretreated with 5 μM DRAQ5™ according to manufacturer’s instructions before addition of TR-LtxA. After 30 mins, LtxA was found on the surface of the cell, but not in the cytosol. Scale bar = 10 μm