Figure 4. APC-like hybrid neutrophils originate from CD11b⁺CD15^hiCD66b⁺CD10⁻CD16^lo/int progenitors.

(A) Flow cytometric analysis of the expression of CD10 and CD16 on gated live CD11b⁺CD15^hiCD66b⁺ neutrophils isolated from peripheral blood (PBNs) and bone marrow (BMNs) of cancer patients.

(B) Cytospins were made from sorted BMNs at different stages of maturation and stained with the Hema3 Stat Pack Kit (Wright-Giemsa-like stain).

(C) Sorted BMNs at different stages of maturation were differentiated in the presence of IFN-γ (50 pg/ml) and GM-CSF (50 pg/ml) in vitro. Expression of HLA-DR and CD14 markers was analyzed by flow cytometry on CD11b⁺CD15^hiCD66b⁺ BMNs.

(D) Cytomorphology of APC-like HLA-DR⁺ hybrid neutrophils differentiated from the sorted populations of BMNs at different stages of maturation.

Representative results from 1 of 4 experiments are shown in (A–D). Scale bar, 10 µm. See also Figure S4.