(A) The proliferation and IFN-γ production of anti-CD3 Abs stimulated autologous T cells in the presence of BM-derived canonical and hybrid neutrophils differentiated with hybrid-inducing TCM or IFN-γ (50 pg/ml) and GM-CSF (50 pg/ml).
(B) Summary results of autologous T cell proliferation (top graph) and IFN-γ production (bottom graph) in the presence of canonical and hybrid neutrophils. Data are presented as a ratio (CD3 cells+CD15hi)/(CD3) (n = 8,Wilcoxon matched-pairs rank test).
(C) The proliferation of CFSE-labeled autologous PBMCs cultured with hybrid BMNs with different level of PD-L1 expression in the presence (bottom panel) or absence PD-L1 blocking Abs (5 µg/ml) (top panel). PD-L1−/lo/hiHLA-DR+ hybrid neutrophils were differentiated with GM-CSF (50 pg/ml) and the increasing doses of IFN-γ.
(D) The proliferation of allogeneic T cells from healthy donors in the presence of APC-like hybrid neutrophils in mixed lymphocyte reaction (MLR).
Representative results from 1of 6 experiments are shown in (C) and (D).
See also Figure S5.