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. Author manuscript; available in PMC: 2017 Jul 11.
Published in final edited form as: Cancer Cell. 2016 Jun 30;30(1):120–135. doi: 10.1016/j.ccell.2016.06.001

Figure 6. APC-like hybrid neutrophils stimulate antigen non-specific T cell responses.

Figure 6

(A) The proliferation and IFN-γ production of anti-CD3 Abs stimulated autologous T cells in the presence of BM-derived canonical and hybrid neutrophils differentiated with hybrid-inducing TCM or IFN-γ (50 pg/ml) and GM-CSF (50 pg/ml).

(B) Summary results of autologous T cell proliferation (top graph) and IFN-γ production (bottom graph) in the presence of canonical and hybrid neutrophils. Data are presented as a ratio (CD3 cells+CD15hi)/(CD3) (n = 8,Wilcoxon matched-pairs rank test).

(C) The proliferation of CFSE-labeled autologous PBMCs cultured with hybrid BMNs with different level of PD-L1 expression in the presence (bottom panel) or absence PD-L1 blocking Abs (5 µg/ml) (top panel). PD-L1−/lo/hiHLA-DR+ hybrid neutrophils were differentiated with GM-CSF (50 pg/ml) and the increasing doses of IFN-γ.

(D) The proliferation of allogeneic T cells from healthy donors in the presence of APC-like hybrid neutrophils in mixed lymphocyte reaction (MLR).

Representative results from 1of 6 experiments are shown in (C) and (D).

See also Figure S5.