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. 2016 Jun 3;55(30):8561–8565. doi: 10.1002/anie.201601984

Figure 5.

Figure 5

STK4 depletion drives MCF‐10A cells to be more invasive. A) Co‐staining of F‐actin and STK4 in MCF‐10A cells (scale bar: 5 μm). B) Western blot analysis of WT and sgSTK4‐modified MCF‐10A cells. C) Real‐time PCR to measure gene expression in STK4 KO MCF‐10A cells. Cells expressing FLAG‐Cas9 only were used as the control. D) STK4 depletion promotes invasion of MCF‐10A cells, as detected by a Matrigel‐coated transwell invasion assay (scale bar: 2 mm). Quantification data is shown. E) Mammosphere formation in MCF‐10A/Cas9 only and MCF‐10A/STK4 KO cells (scale bar: 50 μm). Quantification data are shown. F) The ratio of CD44high/CD24low stem‐like cells in MCF‐10A expressing FLAG‐Cas9 only and in STK4 KO MCF‐10A cells.