. Author manuscript; available in PMC: 2017 Jul 14.

Published in final edited form as: J Med Chem. 2016 Jun 17;59(13):6136–6148. doi: 10.1021/acs.jmedchem.6b00040

Table 6.

Biochemical inhibition of RT-associated RNase H and the antiviral correlation

Compound	RNase H	INST	CPE	MAGI
Compound	IC₅₀ (μM)^a	IC₅₀ (μM)^a	EC₅₀ (μM)^b	EC₅₀ (μM)^c
26	0.061 ± 0.004	0.080 ± 0.007	0.83	3.7 ± 0.3
30	0.042 ± 0.012	0.033 ± 0.004	0.061	0.30 ± 0.01
38	0.011 ± 0.003	0.049 ± 0.004	0.033	0.09 ± 0.02
40	0.010 ± 0.006	0.068 ± 0.011	0.032	0.07 ± 0.02
42	0.026 ± 0.009	0.077 ± 0.011	0.049	0.30 ± 0.06
44	0.022 ± 0.009	0.12 ± 0.021	0.19	0.80 ± 0.2
45	0.029 ± 0.015	0.021 ± 0.002	0.015	0.30 ± 0.09
46	0.020 ± 0.010	0.051 ± 0.013	0.15	1.3 ± 0.2
3	>10	0.068 ± 0.01	--	0.020 ± 0.002
1	>10	0.65 ± 0.14	0.023	0.030 ± 0.008

Concentration with 50% enzyme inhibition, expressed as mean ± standard deviation from three independent experiments.

Concentration of a compound inhibiting virus replication by 50%.

Concentration of a compound inhibiting virus replication by 50%, expressed as mean ± standard deviation from three independent experiments.